The lab claims fixation will cause the dyes to leak from the cells. I do think the belief is always that fixation may perhaps problems cell membranes. I did find a paper which describes fixation using methanol or acetic acid, just after staining by using a DCFDA derivative. DCFDA could https://2-fdck-hcl92467.creacionblog.com/29242176/everything-about-2-fdck-hcl